Mycobacterium phage Techage
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Detailed Information for Phage Techage
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByIsabella Kim
Year Found2017
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.232721 N, 80.409856 W Map
Discovery NotesThis phage was found right in front of the Chase Apartments in downtown Blacksburg, VA. During DNA sequencing, it was discovered that Techage was mixed with another phage. Brianna Chop and Cameron Myers separated the two phages in the lab.
Naming NotesSince I found this phage at Virginia Tech, I decided to combine the two names together to create "Techage"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage435
Genome length (bp)47094
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
Plaque NotesMedium plaques with a clear center and cloudy edge.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Techage_1
(terminase small subunit)

Techage_2
(terminase large subunit)

Techage_3
(portal protein)

Techage_4
(capsid maturation protease)

Techage_5
(scaffolding protein)

Techage_6
(major capsid protein)

Techage_7

Techage_8
(head-to-tail adaptor)

Techage_9
(head-to-tail stopper)

Techage_10

Techage_11

Techage_12

Techage_13
(tail terminator)

Techage_14
(major tail protein)

Techage_15
(tail assembly chaperone)

Techage_16
(tail assembly chaperone)

Techage_17
(tape measure protein)

Techage_18
(minor tail protein)

Techage_19
(minor tail protein)

Techage_20
(minor tail protein)

Techage_21
(minor tail protein)

Techage_22
(minor tail protein)

Techage_23

Techage_24

Techage_25

Techage_26
(lysin A)

Techage_27
(lysin B)

Techage_28
(holin)

Techage_29

Techage_30
(integrase)

Techage_31
(immunity repressor)

Techage_32
(excise)

Techage_33

Techage_34
(antirepressor)

Techage_35

Techage_36

Techage_37

Techage_38

Techage_39

Techage_40

Techage_41

Techage_42

Techage_43

Techage_44

Techage_45
(RecE-like exonuclease)

Techage_46
(RecT-like ssDNA binding protein)

Techage_47

Techage_48
(FtsK-like DNA translocase)

Techage_49
(RusA-like resolvase)

Techage_50

Techage_51

Techage_52

Techage_53

Techage_54

Techage_55

Techage_56
(helix-turn-helix DNA binding domain protein)

Techage_57

Techage_58
(DNA methylase)

Techage_59

Techage_60

Techage_61
(DNA methylase)

Techage_62

Techage_63
(helix-turn-helix DNA binding domain protein)

Techage_64

Techage_65

Techage_66
(helix-turn-helix DNA binding domain protein)

Techage_67

Techage_68

Techage_69

Techage_70

Techage_71

Techage_72

Techage_73

Techage_74

Techage_75

Techage_76

Techage_77

Techage_78

Techage_79
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK919480
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 54
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload