Mycobacterium phage Mangethe
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Detailed Information for Phage Mangethe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTHEMBA ZWANE
Year Found2015
Location FoundDURBAN, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.8675 S, 30.9808 E Map
Discovery NotesThe Soil sample was collected at the University of Kwazulu-Natal at Westville campus, opposite the Microbiology undergraduate laboratory. next to the environmental undergraduate lab.
Naming NotesThe phage was name after My Last name Is Zwane So naming the phage after myself “Mangethe” was very Important , clan names are family names which are considered more important than surnames among Zulu people, each Zulu person can trace their family history back to a specific male ancestor. Hopefully i'll use my new phage "Mangethe" to trace back some of the phages which are closely related to it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 2, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1939
Genome length (bp)47612
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.4%
Sequencing Notessame length but 1 nucleotide difference from Majeke
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Lysogeny NotesThe phage was immune to the lysogen Cjw1 & BigNuz
Other Cluster Members
Annotating InstitutionUniversity of Kwazulu-Natal
Annotation StatusIn GenBank
Plaque NotesMost of the Plaque morphology were Clear and small in size, Circular radial in shape and some were lawn around them.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Mangethe_1
(terminase small subunit)

Mangethe_2
(terminase large subunit)

Mangethe_3
(portal protein)

Mangethe_4
(capsid maturation protease)

Mangethe_5
(scaffolding protein)

Mangethe_6
(major capsid protein)

Mangethe_7

Mangethe_8
(head-to-tail adaptor)

Mangethe_9
(head-to-tail stopper)

Mangethe_10

Mangethe_11

Mangethe_12

Mangethe_13
(tail terminator)

Mangethe_14
(major tail protein)

Mangethe_15
(tail assembly chaperone)

Mangethe_16
(tail assembly chaperone)

Mangethe_17
(tape measure protein)

Mangethe_18
(minor tail protein)

Mangethe_19
(minor tail protein)

Mangethe_20
(minor tail protein)

Mangethe_21
(minor tail protein)

Mangethe_22

Mangethe_23

Mangethe_24

Mangethe_25

Mangethe_26
(lysin A)

Mangethe_27
(lysin B)

Mangethe_28
(holin)

Mangethe_29

Mangethe_30
(integrase)

Mangethe_31
(immunity repressor)

Mangethe_32
(helix-turn-helix DNA binding protein)

Mangethe_33

Mangethe_34
(antirepressor)

Mangethe_35

Mangethe_36

Mangethe_37

Mangethe_38

Mangethe_39

Mangethe_40

Mangethe_41

Mangethe_42

Mangethe_43

Mangethe_44

Mangethe_45

Mangethe_46
(exonuclease)

Mangethe_47
(ssDNA binding protein)

Mangethe_48

Mangethe_49
(Ftsk-like DNA translocase)

Mangethe_50
(RusA-like DNA endonuclease)

Mangethe_51

Mangethe_52

Mangethe_53

Mangethe_54

Mangethe_55

Mangethe_56
(helix-turn-helix DNA binding protein)

Mangethe_57

Mangethe_58

Mangethe_59

Mangethe_60

Mangethe_61
(helix-turn-helix DNA binding protein)

Mangethe_62

Mangethe_63
(DNA methylase)

Mangethe_64

Mangethe_65
(helix-turn-helix DNA binding protein)

Mangethe_66

Mangethe_67

Mangethe_68
(helix-turn-helix DNA binding domain protein)

Mangethe_69

Mangethe_70

Mangethe_71

Mangethe_72

Mangethe_73

Mangethe_74

Mangethe_75

Mangethe_76

Mangethe_77

Mangethe_78

Mangethe_79

Mangethe_80

Mangethe_81
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016499
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload