Mycobacterium phage Majeke
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Detailed Information for Phage Majeke
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySiphenkosi Majeke
Year Found2015
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates29.817085 S, 30.941812 E Map
Discovery NotesThe soil sample was taken under a tree in E-block walkway
Naming NotesThe name of the phage comes from my last
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 7, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2104
Genome length (bp)47612
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Lysogeny Notesimmunity was observed on lysogen Rey and BigNuz
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes-The plaques were circular ring which looked like halos on the agar plate
-The halo shape formed after the 48 hours of incubation at 37 degrees Celsius.
-the size of the plaque was relatively medium size but a small portion of the plaques were smaller
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Majeke_1
(terminase small subunit)

Majeke_2
(terminase large subunit)

Majeke_3
(portal protein)

Majeke_4
(capsid maturation protease)

Majeke_5
(scaffolding protein)

Majeke_6
(major capsid protein)

Majeke_7

Majeke_8
(head-to-tail adaptor)

Majeke_9
(head-to-tail stopper)

Majeke_10

Majeke_11

Majeke_12

Majeke_13
(tail terminator)

Majeke_14
(major tail protein)

Majeke_15
(tail assembly chaperone)

Majeke_16
(tail assembly chaperone)

Majeke_17
(tape measure protein)

Majeke_18
(minor tail protein)

Majeke_19
(minor tail protein)

Majeke_20
(minor tail protein)

Majeke_21
(minor tail protein)

Majeke_22

Majeke_23

Majeke_24

Majeke_25

Majeke_26
(lysin A)

Majeke_27
(lysin B)

Majeke_28
(holin)

Majeke_29

Majeke_30
(integrase)

Majeke_31
(immunity repressor)

Majeke_32
(helix-turn-helix DNA binding protein)

Majeke_33

Majeke_34
(antirepressor)

Majeke_35

Majeke_36

Majeke_37

Majeke_38

Majeke_39

Majeke_40

Majeke_41

Majeke_42

Majeke_43

Majeke_44

Majeke_45

Majeke_46
(exonuclease)

Majeke_47
(ssDNA binding protein)

Majeke_48

Majeke_49
(FtsK-like DNA translocase)

Majeke_50
(RusA-like DNA endonuclease)

Majeke_51

Majeke_52

Majeke_53

Majeke_54

Majeke_55

Majeke_56
(helix-turn-helix DNA binding protein)

Majeke_57

Majeke_58

Majeke_59

Majeke_60

Majeke_61
(helix-turn-helix DNA binding protein)

Majeke_62

Majeke_63
(DNA methylase)

Majeke_64

Majeke_65
(helix-turn-helix DNA binding protein)

Majeke_66

Majeke_67

Majeke_68
(helix-turn-helix DNA binding domain protein)

Majeke_69

Majeke_70

Majeke_71

Majeke_72

Majeke_73

Majeke_74

Majeke_75

Majeke_76

Majeke_77

Majeke_78

Majeke_79

Majeke_80

Majeke_81
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF472894
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload