Mycobacterium phage Shipwreck
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Detailed Information for Phage Shipwreck
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAudrey Tolbert
Year Found2012
Location FoundShippensburg, PA USA
Finding InstitutionBucknell University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.01005 N, 77.506444 W Map
Discovery NotesThe sample was taken from the surface of rocky, dry soil from a garden. It was most likely topsoil originally but was not recently fertilized.
Naming NotesThis name was chosen because the phage was isolated from soil in Shippensburg
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 12, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage350
Sanger Finishing Reactions2
Genome length (bp)48670
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques were bullseyes, with a very clear center surrounded by an opaque ring. The size of the opaque ring changed depending on the titer. As the concentration of phage increases, the diameter of the ring decreased.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Shipwreck_1
(terminase small subunit)

Shipwreck_2
(terminase large subunit)

Shipwreck_3
(portal protein)

Shipwreck_4
(capsid maturation protease)

Shipwreck_5
(scaffolding protein)

Shipwreck_6
(major capsid protein)

Shipwreck_7

Shipwreck_8
(head-to-tail adaptor)

Shipwreck_9
(head-to-tail stopper)

Shipwreck_10

Shipwreck_11

Shipwreck_12
(tail terminator)

Shipwreck_13
(major tail subunit)

Shipwreck_14
(tail assembly chaperone)

Shipwreck_15
(tail assembly chaperone)

Shipwreck_16
(tape measure protein)

Shipwreck_17
(minor tail protein)

Shipwreck_18
(minor tail protein)

Shipwreck_19
(minor tail protein)

Shipwreck_20
(minor tail protein)

Shipwreck_21
(minor tail protein)

Shipwreck_22

Shipwreck_23

Shipwreck_24

Shipwreck_25

Shipwreck_26

Shipwreck_27

Shipwreck_28
(lysin A)

Shipwreck_29
(lysin B)

Shipwreck_30

Shipwreck_31

Shipwreck_32
(integrase)

Shipwreck_33
(immunity repressor)

Shipwreck_34
(HTH DNA binding domain protein)

Shipwreck_35

Shipwreck_36
(antirepressor)

Shipwreck_37

Shipwreck_38

Shipwreck_39

Shipwreck_40

Shipwreck_41

Shipwreck_42

Shipwreck_43

Shipwreck_44

Shipwreck_45

Shipwreck_46

Shipwreck_47
(HNH endonuclease)

Shipwreck_48

Shipwreck_49
(exonuclease)

Shipwreck_50
(ssDNA binding protein)

Shipwreck_51

Shipwreck_52

Shipwreck_53
(translocase)

Shipwreck_54
(endonuclease)

Shipwreck_55

Shipwreck_56

Shipwreck_57
(DNA methylase)

Shipwreck_58

Shipwreck_59

Shipwreck_60

Shipwreck_61
(HTH DNA binding domain protein)

Shipwreck_62

Shipwreck_63

Shipwreck_64

Shipwreck_65

Shipwreck_66

Shipwreck_67

Shipwreck_68

Shipwreck_69

Shipwreck_70

Shipwreck_71

Shipwreck_72
(peptidase)

Shipwreck_73

Shipwreck_74

Shipwreck_75

Shipwreck_76

Shipwreck_77

Shipwreck_78

Shipwreck_79

Shipwreck_80

Shipwreck_81

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU985090
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 33
Pitt Freezer Box Grid# 59
Available Files
Plaque PictureDownload
EM PictureDownload