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Ixel (EA11) Nebulous (EA5) Gandalph (F1) Coco12 (F1) Rebel (N)
Mycobacterium phage Necropolis
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Detailed Information for Phage Necropolis
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLauren Strickland
Year Found2018
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.2336 N, 80.4076 W Map
Discovery NotesSample found on a slope facing NW, beside stairs to a cemetery, across the street from a cemetery
Naming NotesNecropolis means "City of the Dead". See discovery notes
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1057
Genome length (bp)46263
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
Plaque NotesSmall, translucent
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Necropolis_1
(terminase small subunit)

Necropolis_2
(terminase large subunit)

Necropolis_3
(portal protein)

Necropolis_4
(capsid maturation protease)

Necropolis_5
(scaffolding protein)

Necropolis_6
(major capsid protein)

Necropolis_7

Necropolis_8
(head-to-tail adaptor)

Necropolis_9
(head-to tail stopper)

Necropolis_10

Necropolis_11

Necropolis_12

Necropolis_13
(tail terminator)

Necropolis_14
(major tail protein)

Necropolis_15
(tail assembly chaperone)

Necropolis_16
(tail assembly chaperone)

Necropolis_17
(tape measure protein)

Necropolis_18
(minor tail protein)

Necropolis_19
(minor tail protein)

Necropolis_20
(minor tail protein)

Necropolis_21
(minor tail protein)

Necropolis_22

Necropolis_23

Necropolis_24

Necropolis_25

Necropolis_26
(lysin A)

Necropolis_27
(lysin B)

Necropolis_28
(holin)

Necropolis_29

Necropolis_30
(integrase)

Necropolis_31
(immunity repressor)

Necropolis_32

Necropolis_33

Necropolis_34
(antirepressor)

Necropolis_35

Necropolis_36

Necropolis_37

Necropolis_38

Necropolis_39

Necropolis_40

Necropolis_41

Necropolis_42

Necropolis_43

Necropolis_44

Necropolis_45
(RecE-like exonuclease)

Necropolis_46
(RecT-like ssDNA binding protein)

Necropolis_47

Necropolis_48
(FtsK-like DNA translocase)

Necropolis_49
(RusA-like resolvase)

Necropolis_50

Necropolis_51

Necropolis_52
(DNA methylase)

Necropolis_53

Necropolis_54
(methyltransferase)

Necropolis_55

Necropolis_56
(helix-turn-helix DNA binding domain protein)

Necropolis_57

Necropolis_58

Necropolis_59

Necropolis_60
(helix-turn-helix DNA binding domain protein)

Necropolis_61

Necropolis_62

Necropolis_63
(helix-turn-helix DNA binding domain protein)

Necropolis_64

Necropolis_65

Necropolis_66

Necropolis_67

Necropolis_68

Necropolis_69

Necropolis_70

Necropolis_71

Necropolis_72

Necropolis_73

Necropolis_74

Necropolis_75

Necropolis_76
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK937604
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 80
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload