Mycobacterium phage Tortellini
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Detailed Information for Phage Tortellini
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJessica Schipper and Christian Erchinger
Year Found2013
Location FoundBoulder, CO USA
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.998704 N, 105.252092 W Map
Discovery NotesUnder a tree by Stearns West in Williams Village. 9/15/13 9:45pm
Naming NotesStarted with the name Apollo and found out the name was already taken. Then we tried to use the name Nagini but, there was copyright issues. So we debated between Tortellini and Flood.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 18, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage154
Genome length (bp)49658
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCGCCCGC
GC Content65.8%
Sequencing NotesThis phage was sequenced as part of a pool of phages. The sequence was matched to the physical sample and name by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesTurbid plaque with light halo, Size(mm): 1.5mm, Incubation time and temperature: Two days in 37°C heat
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Tortellini_1
(terminase small subunit)

Tortellini_2
(terminase large subunit)

Tortellini_3
(portal protein)

Tortellini_4
(capsid maturation protease)

Tortellini_5
(scaffolding protein)

Tortellini_6
(major capsid protein)

Tortellini_7

Tortellini_8
(head-to-tail adaptor)

Tortellini_9
(head-to-tail stopper)

Tortellini_10

Tortellini_11

Tortellini_12
(tail terminator)

Tortellini_13
(major tail protein)

Tortellini_14
(tail assembly chaperone)

Tortellini_15
(tail assembly chaperone)

Tortellini_16
(tape measure protein)

Tortellini_17
(minor tail protein)

Tortellini_18
(minor tail protein)

Tortellini_19
(minor tail protein)

Tortellini_20
(minor tail protein)

Tortellini_21
(minor tail protein)

Tortellini_22
(minor tail protein)

Tortellini_23

Tortellini_24

Tortellini_25

Tortellini_26
(lysin A)

Tortellini_27
(holin)

Tortellini_28

Tortellini_29
(DNA methylase)

Tortellini_30

Tortellini_31

Tortellini_32
(integrase)

Tortellini_33
(helix-turn-helix DNA binding domain protein)

Tortellini_34
(peptidase)

Tortellini_35

Tortellini_36

Tortellini_37
(immunity repressor)

Tortellini_38
(CRO protein)

Tortellini_39

Tortellini_40
(antirepressor)

Tortellini_41
(helix-turn-helix DNA binding domain protein)

Tortellini_42

Tortellini_43

Tortellini_44

Tortellini_45

Tortellini_46

Tortellini_47

Tortellini_48

Tortellini_49
(WhiB family transcription factor)

Tortellini_50

Tortellini_51

Tortellini_52
(recombination directionality factor)

Tortellini_53

Tortellini_54

Tortellini_55
(exonuclease)

Tortellini_56

Tortellini_57
(DNA polymerase III sliding clamp beta)

Tortellini_58

Tortellini_59
(resolvase)

Tortellini_60

Tortellini_61
(helix-turn-helix DNA binding domain protein)

Tortellini_62

Tortellini_63

Tortellini_64

Tortellini_65

Tortellini_66

Tortellini_67
(HNH endonuclease)

Tortellini_68

Tortellini_69

Tortellini_70

Tortellini_71
(helix-turn-helix DNA binding domain protein)

Tortellini_72

Tortellini_73

Tortellini_74

Tortellini_75
(HNH endonuclease)

Tortellini_76
(polynucleotide kinase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX648391
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2013UCBOTortelliniSE
SEA Lysate Titer 7.7x10^10 pfu/mL
Date of SEA Lysate Titering Nov 8, 2013
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload