Mycobacterium phage Bunnies
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Detailed Information for Phage Bunnies
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChis Bartlett
Year Found2013
Location FoundLawrenceville, GA USA
Finding InstitutionGeorgia Gwinnett College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates34.025 N, 83.944 W Map
Discovery NotesWent into my backyard next to my creek, dug up some soil. under some trees in the shade.
Naming NotesI've always wanted a bunch of bunny rabbits so i figured my phages are my bunnies
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 21, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage1084
Sanger Finishing Reactions2
Genome length (bp)48822
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCGCCCGA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionNeumann University
Annotation StatusIn GenBank
Plaque Notesplaques ranging from .3-.5 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Bunnies_1
(terminase small subunit)

Bunnies_2
(terminase large subunit)

Bunnies_3
(portal protein)

Bunnies_4
(capsid maturation protease)

Bunnies_5
(scaffolding protein)

Bunnies_6
(major capsid protein)

Bunnies_7

Bunnies_8
(head-to-tail adaptor)

Bunnies_9
(head-to-tail stopper)

Bunnies_10

Bunnies_11

Bunnies_12

Bunnies_13
(tail terminator)

Bunnies_14
(major tail protein)

Bunnies_15
(tail assembly chaperone)

Bunnies_16
(tail assembly chaperone)

Bunnies_17
(tape measure protein)

Bunnies_18
(minor tail protein)

Bunnies_19
(minor tail protein)

Bunnies_20

Bunnies_21
(minor tail protein)

Bunnies_22

Bunnies_23

Bunnies_24

Bunnies_25
(membrane protein)

Bunnies_26
(lysin A)

Bunnies_27
(lysin B)

Bunnies_28

Bunnies_29
(membrane protein)

Bunnies_30
(tyrosine integrase)

Bunnies_31
(immunity repressor)

Bunnies_32
(excise)

Bunnies_33

Bunnies_34
(antirepressor)

Bunnies_35

Bunnies_36

Bunnies_37

Bunnies_38

Bunnies_39

Bunnies_40

Bunnies_41

Bunnies_42

Bunnies_43

Bunnies_44

Bunnies_45

Bunnies_46
(RecE-like exonuclease)

Bunnies_47
(RecT-like ssDNA binding protein)

Bunnies_48

Bunnies_49

Bunnies_50
(FtsK-like DNA translocase)

Bunnies_51
(RusA-like resolvase)

Bunnies_52

Bunnies_53

Bunnies_54
(membrane protein)

Bunnies_55
(queuine tRNA-ribosyltransferase)

Bunnies_56

Bunnies_57

Bunnies_58
(DNA methylase)

Bunnies_59

Bunnies_60
(DNA methylase)

Bunnies_61

Bunnies_62
(helix-turn-helix DNA binding domain protein)

Bunnies_63

Bunnies_64

Bunnies_65
(HNH endonuclease)

Bunnies_66

Bunnies_67

Bunnies_68
(helix-turn-helix DNA binding domain protein)

Bunnies_69

Bunnies_70

Bunnies_71
(helix-turn-helix DNA binding domain protein)

Bunnies_72

Bunnies_73

Bunnies_74

Bunnies_75

Bunnies_76
(ribbon-helix-helix DNA binding domain protein)

Bunnies_77

Bunnies_78

Bunnies_79

Bunnies_80

Bunnies_81
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN096356
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.1x10^10
Pitt Freezer Box# 3
Pitt Freezer Box Grid# A2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload