Mycobacterium phage Dynamo
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Detailed Information for Phage Dynamo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChristina Holder, Kaitlyn Menard, Brady Tyler
Year Found2021
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.46402 N, 94.728226 W Map
Discovery NotesAt 9:56 AM CET on 8/26/2021, our lab group (Christina Holder, Kaitlyn Menard, and Brady Tyler) gathered a soil sample beneath a tree near the west-central door of the Glaske Center at LeTourneau University. Brady Tyler used a plastic spoon to dig out an area approximately 8.2 cm x 6.3cm wide and 5.5 cm deep to collect 12 mL of soil. The weather was sunny with clear skies, and the temperature was approximately 29.4°C. The soil was dry and had small, dried-out roots around the dig site.
Naming NotesOur phage was named after the prior complications we experienced with incubation and temperature control. Several of the plates resulting from purifying the phage were heat-shocked during incubation, and we had to start the process over multiple times. A 'Dynamo' is an object that generates force from heat transfer, so we decided that it fit well with the theme of our isolation and research of our phage.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe phage exhibited two distinct plaque morphologies, despite sufficient purification. The larger plaques had more crisp margins, and had an average diameter of 1.25 mm. The smaller plaques had more fuzzy edges, and they exhibited an average diameter of 0.612 mm. Both were turbid, and the overall average diameter of the plaques was 0.931 mm.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload