Mycobacterium phage Dynamo
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Detailed Information for Phage Dynamo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChristina Holder, Kaitlyn Menard, Brady Tyler
Year Found2021
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.46402 N, 94.728226 W Map
Discovery NotesAt 9:56 AM CET on 8/26/2021, our lab group (Christina Holder, Kaitlyn Menard, and Brady Tyler) gathered a soil sample beneath a tree near the west-central door of the Glaske Center at LeTourneau University. Brady Tyler used a plastic spoon to dig out an area approximately 8.2 cm x 6.3cm wide and 5.5 cm deep to collect 12 mL of soil. The weather was sunny with clear skies, and the temperature was approximately 29.4°C. The soil was dry and had small, dried-out roots around the dig site.
Naming NotesOur phage was named after the prior complications we experienced with incubation and temperature control. Several of the plates resulting from purifying the phage were heat-shocked during incubation, and we had to start the process over multiple times. A 'Dynamo' is an object that generates force from heat transfer, so we decided that it fit well with the theme of our isolation and research of our phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 1, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1499
Genome length (bp)46673
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.2%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Plaque NotesThe phage exhibited two distinct plaque morphologies, despite sufficient purification. The larger plaques had more crisp margins, and had an average diameter of 1.25 mm. The smaller plaques had more fuzzy edges, and they exhibited an average diameter of 0.612 mm. Both were turbid, and the overall average diameter of the plaques was 0.931 mm.
Has been Phamerated?Yes
Gene List

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP434454
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 134
Pitt Freezer Box Grid# C12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload