Mycobacterium phage Polkaroo
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Detailed Information for Phage Polkaroo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAmy Stewart
Year Found2022
Location FoundOttawa, Canada
Finding InstitutionUniversity of Ottawa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature19°C
GPS Coordinates45.240719 N, 75.38586 W Map
Discovery NotesFound in a wet garden bed planted outside of the uOttawa Medical campus at RGN
Naming NotesNamed after the mysterious Polkaroo from "The Polkadot Door" TV show as it was a creature that was hard to catch.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1920
Genome length (bp)49084
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Ottawa
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesSmall plaques, clear, small halo.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload