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Mycobacterium phage Donovan
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Detailed Information for Phage Donovan
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChantelle Willette and Seth Stevens
Year Found2012
Location FoundMineral Wells, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates32.75915 N, 98.069478 W Map
Discovery NotesArea watered nightly
96 degrees Fahrenheit
3 cm below surface
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage660
Sanger Finishing Reactions4
Genome length (bp)47162
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCCCCCGA
GC Content67.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall, clear plaques. Generally no larger than 1 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF841477
Refseq NumberNC_023552
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 2E11 pfu/mL
Date of SEA Lysate Titering Oct 24, 2012
Available Files
Restriction Digest PictureDownload