Mycobacterium phage Malithi
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Detailed Information for Phage Malithi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLungile Marcia Mbambo
Year Found2013
Location FoundDurban (KwaMashu), South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.835029 S , 31.014277 E Map
Discovery Notesthe soil sample was taken from KwaMashu township garden, the phage in lower dilutions(10-1) destroys all bacterial cells, it was observed the on the second day of the course which was excitting.the phage was purified 2 times for EM 3rd times for Titer.
Naming Notesthe name of the phage comes from the finder`s name and the behaviour of the phage to the solid agar surface. Malithi means "queen of war", the phage ate ever cell in the growth medium plate indicating that it is dominating the environment.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 13, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage375
Sanger Finishing Reactions11
Genome length (bp)46870
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCTGCCGCCCGA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notesthe plaques had a smooth surface, not toatally round in shape but some were and grew in different sizes. some of the plaques were bigger than the others while others remained tiny. increasing incubation time results in more clearer plaques
MorphotypeSiphoviridae
Number of Genes80
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKP027200
Refseq NumberNC_026605
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# E8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload