| Detailed Information for Phage Bogie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Spook |
| Found By | Reid Rogers |
| Year Found | 2015 |
| Location Found | Camden, AR USA |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.470016 N, 92.873349 W Map |
| Discovery Notes | Depth: 1.27 cm Moist soil Temp: 33.8889 Celsius Location: Latitude: 33.4700165 Longitude: -92.87334859 Sea Level: 67 m Weather: 24 August 2015 - 30 August 2015 Temperature: Max- 33.8889 degrees Celsius Mean- 23.8889 degrees Celsius Min- 13.8889 degrees Celsius Precipitation: Rained 1.1938 cm on 24 August 2015 25 August 2015 - 30 August 2015 no precipitation occured. Soil Sample Collection Information: . On a North facing slope . 20.32 cm above a pool of stagnant water with film on surface . Flowing creek 243.84 cm from sample location . Collected under green moss with red buds that released a fine red powder . Collected below an oak tree and holly tree . Just above sample site a decomposing oak tree with fungus is present . No human traffic in proximity of sample site . Deer and raccoon traffic around sample site . Asphalt road approximately 32 meters from collection site |
| Naming Notes | Named after the term ghost in phages. i.e(synonym for ghost) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2988 |
| Genome length (bp) | 48639 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Further experiments were done with our phage in order to test its lysogeny. A serial dilution of our HTL was used for a spot test in order to determine which dilution formed the best mesa. The undiluted HTL formed the best mesa. We grew this M. smegmatis (Bogie) on a clean TA plate and tested it on a bacterial lawn for halo formation. A halo formed and the lysogen grew. Therefore, Bogie is a great lysogen candidate. |
| Other Cluster Members |
Click to ViewArib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6) |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small circular plaques with a large halo on the exterior. Hazy center and cloudy edges. Temperate phage resemblance. |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBogie_1 Bogie_2 Bogie_3 Bogie_4 Bogie_5 Bogie_6 Bogie_7 Bogie_8 Bogie_9 Bogie_10 Bogie_11 Bogie_12 Bogie_13 Bogie_14 Bogie_15 Bogie_16 Bogie_17 Bogie_18 Bogie_19 Bogie_20 Bogie_21 Bogie_22 Bogie_23 Bogie_24 Bogie_25 Bogie_26 Bogie_27 Bogie_28 Bogie_29 Bogie_30 Bogie_31 Bogie_32 Bogie_33 Bogie_34 Bogie_35 Bogie_36 Bogie_37 Bogie_38 Bogie_39 Bogie_40 Bogie_41 Bogie_42 Bogie_43 Bogie_44 Bogie_45 Bogie_46 Bogie_47 Bogie_48 Bogie_49 Bogie_50 Bogie_51 Bogie_52 Bogie_53 Bogie_54 Bogie_55 Bogie_56 Bogie_57 Bogie_58 Bogie_59 Bogie_60 Bogie_61 Bogie_62 Bogie_63 Bogie_64 Bogie_65 Bogie_66 Bogie_67 Bogie_68 Bogie_69 Bogie_70 Bogie_71 Bogie_72 Bogie_73 Bogie_74 Bogie_75 Bogie_76 Bogie_77 Bogie_78 Bogie_79 Bogie_80 Bogie_81 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF133446 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6.9 x 10^9 |
| Date of SEA Lysate Titering | Oct 15, 2015 |
| Pitt Freezer Box# | 22 |
| Pitt Freezer Box Grid# | E6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |