| Detailed Information for Phage Boneham |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Bethany Lindsey |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444800 N, 79.952700 W Map |
| Discovery Notes | This phage was found on 1/28/16 in a flower bed outside of the Cathedral of Learning the University of Pittsburgh campus. No plants were currently growing within the bed. The temperature during discovery was 43 degrees celcius and had been cloudy the past 24 hours. There were minimal snow showers around the time of harvesting and there was no snow cover in the flowerbed. |
| Naming Notes | Named after 2004 "Survivor" contestant Rupert Boneham. This phage survived mass contamination of an enrichment so I figured it was appropriate to name it after a fan favorite on the television show where surviving is the means of winning. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1822 |
| Genome length (bp) | 77497 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are ~1mm in diameter and are clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG757155 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
