Detailed Information for Phage Bowden |
Discovery Information |
Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
Found By | Stormie Bowden |
Year Found | 2016 |
Location Found | Denton, TX USA |
Finding Institution | University of North Texas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 33.210659 N, 97.147261 W Map |
Discovery Notes | I took the soil sample at 9:30am on September 12, 2016. I dug approximately half an inch into the ground. It was 61 degrees Fahrenheit, and the soil was slightly moist. |
Naming Notes | Because I did the research to find the phage, I named it after myself. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 7, 2017 |
Sequencing Facility | University of North Texas |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 156 |
Genome length (bp) | 52603 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CGCCACGTCTT |
GC Content | 67.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | BD |
Subcluster | BD2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of North Texas |
Annotation Status | In GenBank |
Plaque Notes | The plaques were clear, circular, and measured 1mm in diameter. |
Morphotype | Siphoviridae |
Number of Genes | 81 |
Number of tRNAs | 1 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK392363 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
4x10^8 pfu/mL |
Date of SEA Lysate Titering |
Nov 7, 2016 |
Pitt Freezer Box# |
36 |
Pitt Freezer Box Grid# |
D7 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |