| Detailed Information for Phage BroPlease |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Faith LaBry & Ahmad Sulaiman |
| Year Found | 2021 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.254578 N, 97.146434 W Map |
| Discovery Notes | -Obtained from my TA's backyard
-Dug one inch under a grassy surface
-Partially cloudy outside |
| Naming Notes | My name came from the fact that I had numerous struggles with obtaining and isolating the phage. Both of my soil samples yielded no sign of phage (so I had to use the TA's soil sample), and my plate titers kept having to get readjusted due to there not being a high enough plate titer. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 6, 2021 |
| Approximate Shotgun Coverage | 656 |
| Genome length (bp) | 50645 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAGCCGTGAT |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Diameter: ≤1mm (ranging from pinpoint to small)
More heterogeneous due to diversity in plaque sizes
Slightly more temperate (cloudy) over lytic (clear)
Most plaques have a small clear center and turbid edges, sort of like a bullseye or large halo
Only the smallest plaques are clear |
| Number of Genes | 76 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON526970 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
143 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
