| Detailed Information for Phage BronnyJr | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Thomas Shaw, Alexander Soderling |
| Year Found | 2025 |
| Location Found | Blacksburg, VA United States of America |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.23 N, 80.45 W Map |
| Discovery Notes | Located in a flower garden behind a apartment complex, underneath newly spread mulch and fertilizer. Surrounding areas had some white substances around, could have been spider webs. |
| Naming Notes | We wanted to make a name relevant to the current times, the phage Lebron was found by a researcher at JMU a while back, and we wanted to relate our phage to a newer and less discussed public figure, ending with us landing with the name BronnyJr. Upon analyzing the Lebron phage, we also noticed it had a significantly longer tail length than ours, making it appropriate for ours to be deemed a junior, even if the two phages aren't the same or related. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 736 |
| Genome length (bp) | 47139 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| End Determination Notes | 5% of the reads were seen with a contamination of Cluster A11. |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArib1 (P1) Atcoo (P1) Bartholomew (P1) Bhagsy (P1) Bogie (P1) BronnyJr (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) Etoile (P1) FirstPlacePfu (P1) Fishburne (P1) GaloreK (P1) Gavriela (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) PeanutPie (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6) |
| Annotating Institution | Virginia Tech |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques were medium to large in size, and were very prolific, even at high dilution levels. |
| Morphotype | Siphoviridae |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBronnyJr_1 BronnyJr_2 BronnyJr_3 BronnyJr_4 BronnyJr_5 BronnyJr_6 BronnyJr_7 BronnyJr_8 BronnyJr_9 BronnyJr_10 BronnyJr_11 BronnyJr_12 BronnyJr_13 BronnyJr_14 BronnyJr_15 BronnyJr_16 BronnyJr_17 BronnyJr_18 BronnyJr_19 BronnyJr_20 BronnyJr_21 BronnyJr_22 BronnyJr_23 BronnyJr_24 BronnyJr_25 BronnyJr_26 BronnyJr_27 BronnyJr_28 BronnyJr_29 BronnyJr_30 BronnyJr_31 BronnyJr_32 BronnyJr_33 BronnyJr_34 BronnyJr_35 BronnyJr_36 BronnyJr_37 BronnyJr_38 BronnyJr_39 BronnyJr_40 BronnyJr_41 BronnyJr_42 BronnyJr_43 BronnyJr_44 BronnyJr_45 BronnyJr_46 BronnyJr_47 BronnyJr_48 BronnyJr_49 BronnyJr_50 BronnyJr_51 BronnyJr_52 BronnyJr_53 BronnyJr_54 BronnyJr_55 BronnyJr_56 BronnyJr_57 BronnyJr_58 BronnyJr_59 BronnyJr_60 BronnyJr_61 BronnyJr_62 BronnyJr_63 BronnyJr_64 BronnyJr_65 BronnyJr_66 BronnyJr_67 BronnyJr_68 BronnyJr_69 BronnyJr_70 BronnyJr_71 BronnyJr_72 BronnyJr_73 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
