| Detailed Information for Phage Caliburn |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Former names | Excalibur |
| Found By | Danial Jahangir Ali and Sekinat Yeside Quadri |
| Year Found | 2014 |
| Location Found | Grapevine, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.887056 N, 97.094472 W Map |
| Discovery Notes | The soil came from a backyard in Grapevine, TX situated under a tree. The soil had a sand-like texture or feel. There are several small clumps within the sample. The soil sample was collected within the first inch of the top layer. The sample was collected at approximately at 7:00 pm on 8/24/14. The temperature was approximately 80 degrees Fahrenheit outside during the time of sample collection. |
| Naming Notes | The name of the phage is based upon the sword that was in the stone that could only be removed by the true king, Arthur Pendragon. Since the name Excalibur was already taken by a mycobacteriophage, we used Caliburn which is another version of Excalibur in the King Arthur story |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2067 |
| Genome length (bp) | 49949 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| End Determination Notes | Buildup of reads and similarity to Zemlya/Suji/Like |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The size of the plaques vary in size from extremely small to relatively large. The average size of the plaques are 5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT152029 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
C7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
