| Detailed Information for Phage Chucky |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Matthew Choi and Brett Deng |
| Year Found | 2019 |
| Location Found | St. Louis, MO United States of America |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 27°C |
| GPS Coordinates | 38.6367 N, 90.2887 W Map |
| Discovery Notes | The sample was from an area of considerable organic matter present. The soil was moist, and the sample was collected 10 cm beneath the surface. |
| Naming Notes | The Halloween season inspired the name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 29, 2020 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 101 |
| Genome length (bp) | 51594 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGCCAGTGAT |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Small plaques of 2.74mm diameter and medium plaques of 7 mm diametercircular plaques with light pink outlines around the plaques. |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ648032 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.3x10^9 pfu/ml |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
