| Detailed Information for Phage Demosthenes |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Patrick Rimple |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.436419 N, 79.949316 W Map |
| Discovery Notes | This phage was found on the middle of a path down in panther hollow that crosses between the railroad tracks and the pond. It had rained the day before and was around 80 degrees F that day. The soil was the wet top layer that people had stepped on. |
| Naming Notes | Demosthenes is the name a character in the Ender's Game series adopts so that she may write deep philosophical papers. I really liked that so I wanted to name one of my phages after her, and in so I also honor the ancient Greek statesman of the same name who tried to keep Athens free form Macedonia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 3, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1146 |
| Genome length (bp) | 74073 |
| Character of genome ends | Direct Terminal Repeat |
| End Determination Notes | Buildup of reads flanking region of increased coverage. |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and pinprick size to 1mm in size. |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU998242 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
G6 |
| Available Files |
| Final DNAMaster File | Download |