The Actinobacteriophage Database

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Detailed Information for Phage Fishburne
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Alana Guziewicz
Year Found	2011
Location Found	Charleston, SC USA
Finding Institution	College of Charleston
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	32.792778 N, 79.952778 W Map
Discovery Notes	Environmental soil sample taken off of Fishburne Street in downtown Charleston.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 21, 2012
Approximate Shotgun Coverage	210
Genome length (bp)	47109
Character of genome ends	3' Sticky Overhang
Overhang Length	12 bases
Overhang Sequence	CCCGCCCCCCGA
GC Content	67.3%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	P
Subcluster	P1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Arib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Small, Clear, Spread out
Morphotype	Siphoviridae
Number of Genes	77
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Fishburne_1 (terminase small subunit) Fishburne_2 (terminase large subunit) Fishburne_3 (portal protein) Fishburne_4 (capsid maturation protease) Fishburne_5 (scaffolding protein) Fishburne_6 (major capsid protein) Fishburne_7 Fishburne_8 (head-to-tail adaptor) Fishburne_9 (head-to-tail stopper) Fishburne_10 Fishburne_11 Fishburne_12 Fishburne_13 (tail terminator) Fishburne_14 (major tail protein) Fishburne_15 (tail assembly chaperone) Fishburne_16 (tail assembly chaperone) Fishburne_17 (tape measure protein) Fishburne_18 (minor tail protein) Fishburne_19 (minor tail protein) Fishburne_20 (minor tail protein) Fishburne_21 (minor tail protein) Fishburne_22 Fishburne_23 Fishburne_24 Fishburne_25 Fishburne_26 (lysin A) Fishburne_27 (lysin B) Fishburne_28 Fishburne_29 Fishburne_30 (tyrosine integrase) Fishburne_31 (immunity repressor) Fishburne_32 Fishburne_33 Fishburne_34 (antirepressor) Fishburne_35 Fishburne_36 Fishburne_37 Fishburne_38 Fishburne_39 Fishburne_40 Fishburne_41 Fishburne_42 Fishburne_43 Fishburne_44 Fishburne_45 (RecB-like exonuclease/helicase) Fishburne_46 (RecT-like DNA pairing protein) Fishburne_47 Fishburne_48 (FtsK-like DNA translocase) Fishburne_49 (RusA-like resolvase) Fishburne_50 Fishburne_51 Fishburne_52 Fishburne_53 Fishburne_54 Fishburne_55 (helix-turn-helix DNA binding domain protein) Fishburne_56 Fishburne_57 (DNA methylase) Fishburne_58 Fishburne_59 Fishburne_60 Fishburne_61 (RepA-like replication initiator) Fishburne_62 Fishburne_63 Fishburne_64 Fishburne_65 Fishburne_66 Fishburne_67 Fishburne_68 Fishburne_69 Fishburne_70 Fishburne_71 Fishburne_72 Fishburne_73 (ribbon-helix-helix DNA binding domain protein) Fishburne_74 Fishburne_75 Fishburne_76 Fishburne_77
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KC691256
Refseq Number	NC_021302
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2011CCHAfishburneAG
Pitt Freezer Box#	22
Pitt Freezer Box Grid#	7
Available Files
EM Picture	Download

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