| Detailed Information for Phage FiveHead | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Kelly Tang |
| Year Found | 2025 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.053581 N, 118.241475 W Map |
| Discovery Notes | Instructor: Dr. Daniel Velez-Ramirez, Dr. Kris Reddi Teaching Assistant: Praneel Bonthala Undergraduate Assistant(s): Amanda Streva & Jacob Khuu Team #1 Name: Phage Around & Find Out Team #1 Members: Elijah Medrano, Diana Rejon, Kelly Tang, Tiffany Asatryan Sample description: Collected soil was 81 cm above ground level in a large, raised planter that is integrated into the pavement growing a singular tree. The soil was dry. The site of collection is urban with heavy foot traffic as it is proximal to various buildings, such as Los Angeles Town Hall, and a main road. Depth from which sample was taken (in inches or cm): 7 cm Air temperature if sample collected outdoors (in degrees Celsius): 58°F |
| Naming Notes | We named it FiveHead as a play on the term used to describe a large forehead, referencing the phage's notable size and strength. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 18, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 6720 |
| Genome length (bp) | 43309 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAdolin (AZ1) Adumb2043 (AZ1) AEgle (AZ1) AGrandiflora (AZ1) Amyev (AZ1) Asa16 (AZ1) Ascela (AZ1) Berrie (AZ1) Cassia (AZ1) Community (AZ1) Crewmate (AZ1) Cyan (AZ1) DrManhattan (AZ1) DrSierra (AZ1) Elezi (AZ1) Eraser (AZ1) Iter (AZ1) IttyBittyPiggy (AZ1) Janeemi (AZ1) Joemato (AZ1) JohnDoe (AZ1) Jstan (AZ1) JuneStar (AZ1) Kaylissa (AZ1) KeAlii (AZ1) Lego (AZ1) Lizalica (AZ1) London (AZ1) MissSwiss (AZ1) Mudpuppy (AZ1) Niobe (AZ1) Nitro (AZ1) ObiToo (AZ1) Phives (AZ1) Pixelle (AZ1) Powerpuff (AZ1) Pumpkins (AZ1) Reedo (AZ1) Schaffner (AZ1) Simpson (AZ1) Skelbel (AZ1) Subaru (AZ1) Sue2 (AZ1) Tallboi (AZ1) Tbone (AZ1) TforTroy (AZ1) Tian (AZ1) Tuck (AZ1) Turab (AZ1) Tutumahutu (AZ1) VResidence (AZ1) Warda (AZ1) Wildwest (AZ1) Yang (AZ1) YesChef (AZ1) Liebe (AZ2) MaGuCo (AZ2) Maureen (AZ2) Snek (AZ3) Tweety19 (AZ3) Emotion (AZ4) FiveHead (AZ4) JasmineDragon (AZ4) JohnThicc (AZ4) LadyAstra (AZ4) MiniMommy (AZ4) Origami (AZ4) ShakeItOph (AZ4) VroomVroom (AZ4) Ashes (AZ5) Beaupre (AZ5) Cappuccino (AZ5) Donkey (AZ5) Gambol (AZ5) Giorgio (AZ5) Gumpizza (AZ5) Halsey (AZ5) Kalimba (AZ5) Moss (AZ5) Mysterium (AZ5) RockScotty (AZ5) Sabourin (AZ5) Sooty (AZ5) SpecialK (AZ5) Stuu (AZ5) Exile (AZ6) Soondubu (AZ6) AinMach (AZ7) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 1/18/2026) |
| Plaque Notes | pinpoint plaques, circular, clear borders 2-3 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 71 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewFiveHead_1 FiveHead_2 FiveHead_3 FiveHead_4 FiveHead_5 FiveHead_6 FiveHead_7 FiveHead_8 FiveHead_9 FiveHead_10 FiveHead_11 FiveHead_12 FiveHead_13 FiveHead_14 FiveHead_15 FiveHead_16 FiveHead_17 FiveHead_18 FiveHead_19 FiveHead_20 FiveHead_21 FiveHead_22 FiveHead_23 FiveHead_24 FiveHead_25 FiveHead_26 FiveHead_27 FiveHead_28 FiveHead_29 FiveHead_30 FiveHead_31 FiveHead_32 FiveHead_33 FiveHead_34 FiveHead_35 FiveHead_36 FiveHead_37 FiveHead_38 FiveHead_39 FiveHead_40 FiveHead_41 FiveHead_42 FiveHead_43 FiveHead_44 FiveHead_45 FiveHead_46 FiveHead_47 FiveHead_48 FiveHead_49 FiveHead_50 FiveHead_51 FiveHead_52 FiveHead_53 FiveHead_54 FiveHead_55 FiveHead_56 FiveHead_57 FiveHead_58 FiveHead_59 FiveHead_60 FiveHead_61 FiveHead_62 FiveHead_63 FiveHead_64 FiveHead_65 FiveHead_66 FiveHead_67 FiveHead_68 FiveHead_69 FiveHead_70 FiveHead_71 — tRNA FiveHead_72 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
