| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | N. Sullivan, B. Baliraine, S. Merjil, T. Lackman |
| Year Found | 2024 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.468056 N, 94.725417 W Map |
| Discovery Notes | Gavriela was collected at 7:53 a.m. CDT on a cool and cloudy Tuesday morning on August 27, 2024, from the LeTourneau University campus in Longview, Texas, on the West side of Thomas Hall. The ambient temperature was 23.9ºC with a humidity level of 83%. A 3-inch hole was dug with a plastic spoon in an area that was shaded from sunlight. The extracted soil sample was very moist, dark, and rich in organic matter. |
| Naming Notes | Our lab group decided to name our bacteriophage "Gavriela" which means "God is my strength" in Hebrew. Gavriela was chosen because the Lord helped us collect a successful sample of soil to isolate our phage from. We were dependent on God's strength to aid us in all of our endeavors throughout this lab as well as carry us through the trials that we encountered when carrying out the complex procedures. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3804 |
| Genome length (bp) | 47916 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCTGCCGCCCCGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques of Gavriela appeared to be cloudy, and small in size, with an average diameter of 1.12 mm (0.6-1.8; n=15). The plaques were very consistent in size throughout multiple of our plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV251869 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
