| Detailed Information for Phage Gavriela | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | N. Sullivan, B. Baliraine, S. Merjil, T. Lackman |
| Year Found | 2024 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.468056 N, 94.725417 W Map |
| Discovery Notes | Gavriela was collected at 7:53 a.m. CDT on a cool and cloudy Tuesday morning on August 27, 2024, from the LeTourneau University campus in Longview, Texas, on the West side of Thomas Hall. The ambient temperature was 23.9ºC with a humidity level of 83%. A 3-inch hole was dug with a plastic spoon in an area that was shaded from sunlight. The extracted soil sample was very moist, dark, and rich in organic matter. |
| Naming Notes | Our lab group decided to name our bacteriophage "Gavriela" which means "God is my strength" in Hebrew. Gavriela was chosen because the Lord helped us collect a successful sample of soil to isolate our phage from. We were dependent on God's strength to aid us in all of our endeavors throughout this lab as well as carry us through the trials that we encountered when carrying out the complex procedures. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3804 |
| Genome length (bp) | 47916 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCTGCCGCCCCGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Gavriela (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6) |
| Annotating Institution | LeTourneau University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | The plaques of Gavriela appeared to be cloudy, and small in size, with an average diameter of 1.12 mm (0.6-1.8; n=15). The plaques were very consistent in size throughout multiple of our plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGavriela_1 Gavriela_2 Gavriela_3 Gavriela_4 Gavriela_5 Gavriela_6 Gavriela_7 Gavriela_8 Gavriela_9 Gavriela_10 Gavriela_11 Gavriela_12 Gavriela_13 Gavriela_14 Gavriela_15 Gavriela_16 Gavriela_17 Gavriela_18 Gavriela_19 Gavriela_20 Gavriela_21 Gavriela_22 Gavriela_23 Gavriela_24 Gavriela_25 Gavriela_26 Gavriela_27 Gavriela_28 Gavriela_29 Gavriela_30 Gavriela_31 Gavriela_32 Gavriela_33 Gavriela_34 Gavriela_35 Gavriela_36 Gavriela_37 Gavriela_38 Gavriela_39 Gavriela_40 Gavriela_41 Gavriela_42 Gavriela_43 Gavriela_44 Gavriela_45 Gavriela_46 Gavriela_47 Gavriela_48 Gavriela_49 Gavriela_50 Gavriela_51 Gavriela_52 Gavriela_53 Gavriela_54 Gavriela_55 Gavriela_56 Gavriela_57 Gavriela_58 Gavriela_59 Gavriela_60 Gavriela_61 Gavriela_62 Gavriela_63 Gavriela_64 Gavriela_65 Gavriela_66 Gavriela_67 Gavriela_68 Gavriela_69 Gavriela_70 Gavriela_71 Gavriela_72 Gavriela_73 Gavriela_74 Gavriela_75 Gavriela_76 Gavriela_77 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
