| Detailed Information for Phage GirlDinner |
| Discovery Information |
| Isolation Host | Streptomyces baarnensis NRRL B-2842 |
| Found By | Ifeabia Okororie |
| Year Found | 2023 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.21311 N, 97.15483 W Map |
| Discovery Notes | At 1:00 PM, I collected soil under a patch of sunflowers near 217 N Texas Blvd, Denton, Texas (33.21311°N, 97.15483°W). The temperature was 37.78 degrees Celsius (100 degrees Fahrenheit) with a humidity of 37%. I dug about 2 cm under the dry, grainy soil and placed it in a Ziplock bag. |
| Naming Notes | It was a trendy meme at the time. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2334 |
| Genome length (bp) | 50291 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCGTGTCTT |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Cloudy and circular morphology measuring about 1 mm each. |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978788 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^8 |
| Pitt Freezer Box# |
174 |
| Pitt Freezer Box Grid# |
D4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
