| Detailed Information for Phage Hello |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Cecilia Daugherty |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 40.4438 N, 79.9623 W Map |
| Discovery Notes | Phage Hello was isolated from a soil sample found at the Petersen Events Center at approximately 4:00 pm on 9/5/17. The weather was moderate and overcast. The soil was found under a small tree/bush and a building awning. It was slightly damp and pillbugs and small worms were found in/around it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 506 |
| Genome length (bp) | 73453 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 161 bp |
| GC Content | 59.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | After two lysogen tests, neither plate showed mesas growing within the plaques, indicating that it is likely a lytic phage. This aligns with previous speculations made by observing plaques. |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Phage Hello produced clear plaques with round borders that are approximately 0.75mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450435 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
57 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
