| Discovery Information |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Ashlynn Chen |
| Year Found | 2025 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.09899 N, 118.38857 W Map |
| Discovery Notes | Instructor: Dr. Daniel Velez Ramirez
Teaching Assistant: Alexandra (Ally) Aves
Undergraduate Assistant(s): Usha + Pranava
Team #1 Name: Philet Mignon
Team #1 Members: Catie Cable, Ashlynn Chen, Alyson Chou, Ryan Thompson
Sample description: In the shrubs, by some roots, side slope off side of the road in a very exclusive neighborhood where very exclusive residents reside
Depth from which sample was taken (in inches or cm): 8cm deep
Air temperature if sample collected outdoors (in degrees Celsius): 14.44℃ |
| Naming Notes | named after John Wick, but thicker
So thick it clogged 3 filters during the filtration step |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 21, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 8888 |
| Genome length (bp) | 43313 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGCGGCAT |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 1/21/2026) |
| Plaque Notes | clear throughout, crisp edges, no turbidity or halos
Clear, circular, smooth margins, 1-2mm in diameter, through later iterations presented as bullseye of around 1mm diameter
2.1x2.2 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
