| Detailed Information for Phage Kaine | |
| Discovery Information | |
| Isolation Host | Streptomyces bicolor NRRL B-3897 |
| Found By | Andrew Kihwili |
| Year Found | 2024 |
| Location Found | Little Elm, TX United States of America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.15595 N, 96.94897 W Map |
| Discovery Notes | I Collected soil from (33.15595°N, 96.94897°W) at Little Elm Park in Little Elm, Texas. The temperature was approximately 1.11°C with a humidity of 77% at 11:03 pm. The location was near the top of a hill that led down to Lake Lewisville. I chose a location where the soil was exposed and no grass was growing. The soil was muddy/damp due to the weather and location. I dug 1-2cm below the surface and collected a full bag worth of soil. |
| Naming Notes | My brother is an Artist and he goes by the name Nolan Kaine. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5753 |
| Genome length (bp) | 48825 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGTACGTGAT |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BD |
| Subcluster | BD3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAaronocolus (BD1) Asten (BD1) BarryBee (BD1) BeardedLady (BD1) Bovely (BD1) Brataylor (BD1) BryanRecycles (BD1) Caliburn (BD1) Celeste (BD1) Chucky (BD1) Danzina (BD1) Dattran (BD1) Dwayne (BD1) Eddasa (BD1) Ejemplo (BD1) Emaanora (BD1) EnochSoames (BD1) Esperer (BD1) Goby (BD1) Godpower (BD1) Hippo (BD1) Hydra (BD1) Indigo (BD1) Izzy (BD1) Jash (BD1) Katalie (BD1) Lannister (BD1) Legacy (BD1) Leviticus (BD1) Lika (BD1) Lorelei (BD1) Maneekul (BD1) Nabi (BD1) Nanodon (BD1) Nerdos (BD1) Oliynyk (BD1) Ozzie (BD1) OzzyJ (BD1) Phettuccine (BD1) Rana (BD1) RedBear (BD1) Rusticus (BD1) SarahRose (BD1) Snorlax (BD1) South40 (BD1) Sujidade (BD1) SunsetPointe (BD1) TagePhighter (BD1) Toma (BD1) Triste (BD1) TuanPN (BD1) Unstoppable (BD1) Werner (BD1) Whatever (BD1) Yasdnil (BD1) Zemlya (BD1) Alvy (BD2) Amethyst (BD2) Andris (BD2) Animus (BD2) BartholomewSD (BD2) Bowden (BD2) Caelum (BD2) Daudau (BD2) Diane (BD2) ELB20 (BD2) GirlDinner (BD2) Haizum (BD2) Hank144 (BD2) Issmi (BD2) Janus (BD2) Jevington (BD2) Loofah (BD2) Marav (BD2) Nishikigoi (BD2) Omar (BD2) Pablito (BD2) Paedore (BD2) Paolo (BD2) Puginator (BD2) R4 (BD2) SqueakyClean (BD2) Superstar (BD2) Tefunt (BD2) Thestral (BD2) TinaBelcher (BD2) Triumph (BD2) TrvxScott (BD2) Zainub (BD2) Alsaber (BD3) Amela (BD3) Celery (BD3) Conan (BD3) Dexers (BD3) ElGato (BD3) Kaine (BD3) Pavo (BD3) phiCAM (BD3) Provolone (BD3) Saftant (BD3) Speedwell (BD3) Vanseggelen (BD3) Verabelle (BD3) Verse (BD3) Yosif (BD3) BroPlease (BD4) GreenWeasel (BD4) phiHau3 (BD4) StrepC (BD5) Celia (BD6) Itza (BD6) Urza (BD6) VieEnRose (BD6) |
| Annotating Institution | University of North Texas |
| Annotation Status | Being Annotated (Expected completion by 1/2/2025) |
| Plaque Notes | The diameter of the plaques were around 1mm and clear and circular |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2x10^8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
