| Detailed Information for Phage Kaine |
| Discovery Information |
| Isolation Host | Streptomyces bicolor NRRL B-3897 |
| Found By | Andrew Kihwili |
| Year Found | 2024 |
| Location Found | Little Elm, TX United States of America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.15595 N, 96.94897 W Map |
| Discovery Notes | I Collected soil from (33.15595°N, 96.94897°W) at Little Elm Park in Little Elm, Texas. The temperature was approximately 1.11°C with a humidity of 77% at 11:03 pm. The location was near the top of a hill that led down to Lake Lewisville. I chose a location where the soil was exposed and no grass was growing. The soil was muddy/damp due to the weather and location. I dug 1-2cm below the surface and collected a full bag worth of soil. |
| Naming Notes | My brother is an Artist and he goes by the name Nolan Kaine. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5753 |
| Genome length (bp) | 48825 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGTACGTGAT |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The diameter of the plaques were around 1mm and clear and circular |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ723753 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^8 |
| Pitt Freezer Box# |
205 |
| Pitt Freezer Box Grid# |
A6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
