| Detailed Information for Phage Lizalica |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Former names | Alollipop |
| Found By | Kieran Furlong |
| Year Found | 2020 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 11°C |
| GPS Coordinates | 45.38592 N, 75.705088 W Map |
| Discovery Notes | The phage was found in a soil sample located about 2 meters from a pond in Fletcher's Wildlife Garden in Ottawa, Canada. The soil sample was taken 3cm-5cm below the surface in the morning of September 17, 2020. A link to the park's website:
https://ofnc.ca/programs/fletcher-wildlife-garden/fwg-habitats/amphibian-pond |
| Naming Notes | I asked my niece Alice for help naming the bacteriophage. She saw a transmission electron microscope picture of a phage and decided it looked like a lollipop. Lollipop was already taken so we named it Lizalica - the Bosnian translation of lollipop. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 661 |
| Genome length (bp) | 43055 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | In GenBank |
| Plaque Notes | Large clear plaques |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OL549192 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
128 |
| Pitt Freezer Box Grid# |
E11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
