Detailed Information for Phage Mangethe |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | THEMBA ZWANE |
Year Found | 2015 |
Location Found | DURBAN, South Africa |
Finding Institution | University of Kwazulu-Natal |
Program | Mycobacterial Genetics Course, Durban, South Africa |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 29.8675 S, 30.9808 E Map |
Discovery Notes | The Soil sample was collected at the University of Kwazulu-Natal at Westville campus, opposite the Microbiology undergraduate laboratory. next to the environmental undergraduate lab. |
Naming Notes | The phage was name after My Last name Is Zwane So naming the phage after myself “Mangethe” was very Important , clan names are family names which are considered more important than surnames among Zulu people, each Zulu person can trace their family history back to a specific male ancestor. Hopefully i'll use my new phage "Mangethe" to trace back some of the phages which are closely related to it.
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Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Oct 2, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1939 |
Genome length (bp) | 47612 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 12 bases |
Overhang Sequence | CCCGCCCCCCGA |
GC Content | 67.4% |
Sequencing Notes | same length but 1 nucleotide difference from Majeke |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | P |
Subcluster | P1 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | The phage was immune to the lysogen Cjw1 & BigNuz
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Other Cluster Members |
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Annotating Institution | University of Kwazulu-Natal |
Annotation Status | In GenBank |
Plaque Notes | Most of the Plaque morphology were Clear and small in size, Circular radial in shape and some were lawn around them. |
Morphotype | Siphoviridae |
Number of Genes | 81 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK016499 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Not in Pitt Archives |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
Virtual Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |