| Detailed Information for Phage Mangethe |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | THEMBA ZWANE |
| Year Found | 2015 |
| Location Found | DURBAN, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 29.8675 S, 30.9808 E Map |
| Discovery Notes | The Soil sample was collected at the University of Kwazulu-Natal at Westville campus, opposite the Microbiology undergraduate laboratory. next to the environmental undergraduate lab. |
| Naming Notes | The phage was name after My Last name Is Zwane So naming the phage after myself “Mangethe” was very Important , clan names are family names which are considered more important than surnames among Zulu people, each Zulu person can trace their family history back to a specific male ancestor. Hopefully i'll use my new phage "Mangethe" to trace back some of the phages which are closely related to it.
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| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 2, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1939 |
| Genome length (bp) | 47612 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.4% |
| Sequencing Notes | same length but 1 nucleotide difference from Majeke |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | The phage was immune to the lysogen Cjw1 & BigNuz
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| Other Cluster Members |
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| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | Most of the Plaque morphology were Clear and small in size, Circular radial in shape and some were lawn around them. |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK016499 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
