| Detailed Information for Phage Nazo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Naazneen Adam |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.917388 S, 30.899609 E Map |
| Discovery Notes | This phage was isolated from my mothers coriander garden.The soil her was moist but not very wet. the soil was taken from the top of the garden no more than 2cm below the surface. This was one first soil samples that had been enriched but was only plated a week after the soil had been incubated. |
| Naming Notes | The phage was named after myself, as it had taken a lot of hard work to actually find it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 6, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1767 |
| Genome length (bp) | 48870 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCTGCCCCCCCG |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were interesting and cool looking as they could almost not been seen. They were very opaque with a tiny clearing in the center on more that 1mm in diameter, the plaques itself were about 5mm |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX641262 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Final DNAMaster File | Download |