| Detailed Information for Phage Nitro |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Jason Summerfield |
| Year Found | 2020 |
| Location Found | New Haven, CT United States of America |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.334167 N, 72.945556 W Map |
| Discovery Notes | The temperature was around 17 degrees Celsius on the date of discovery 8-31-2020. The soil was dug 2 cm deep and 15 mL of soil was collected. It was found next to a tree and beside a building. The day prior was stormy weather making the soil mud-like. |
| Naming Notes | Named after my dog who recently passed before starting the SEA PHAGES program. He passed in an unfortunate circumstance and his name was Nitro. To honor him, I am naming my phage after him. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 25 |
| Genome length (bp) | 44379 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Southern California |
| Annotation Status | In GenBank |
| Plaque Notes | Small to medium sized plaques. Temperate phage. Titer is 7.1e10. Phage has not yet been sequenced. Results of TEM have yet to be received. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553895 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.1e10 |
| Pitt Freezer Box# |
129 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
