| Detailed Information for Phage ObiToo |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Katia Koziel Ly |
| Year Found | 2021 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 45.244 N, 75.73 W Map |
| Discovery Notes | ObiToo was discovered from a soil sample taken from a compost hole full of sauerkraut. It was extracted using a small scale direct isolation and plaque assays with serial dilutions. |
| Naming Notes | This bacteriophage was named after Obi-Wan Kenobi. Its name is meant to represent "the second Kenobi". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2059 |
| Genome length (bp) | 43969 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | In GenBank |
| Plaque Notes | ObiToo expresses large round plaques with a distinguishing dark halo around the outer edge and a dark bullseye in the center. |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709214 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
133 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
