| Detailed Information for Phage OzzyJ |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Alex Harris & Grant Galasso |
| Year Found | 2015 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 38.648461 N, 90.307196 W Map |
| Discovery Notes | Collected from a patch of mud. It was a very soft and watery mud that had a sort of wet-clay look to it, and it was from a flat surface within a foot of a sidewalk and on the edge of a lawn of grass. The soil was a very yellowy color. |
| Naming Notes | The name was inspired by the disease-fighting amoeba, Osmosis Jones, from the TV show of the same name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 25, 2017 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 186 |
| Genome length (bp) | 51464 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGCCAGTGAT |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were of medium to large size. Edges were well-defined and plaques were clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG757163 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Designator |
OzzyJ |
| SEA Lysate Titer |
2x10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 13, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
