The Actinobacteriophage Database

Mycobacterium phage Phineas

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Detailed Information for Phage Phineas
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Eric Hederstedt
Year Found	2011
Location Found	Holland, MI USA
Finding Institution	Hope College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	42.787639 N, 86.104326 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 28, 2012
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Ion Torrent
Approximate Shotgun Coverage	149
Sanger Finishing Reactions	8
Genome length (bp)	47229
Character of genome ends	3' Sticky Overhang
Overhang Length	12 bases
Overhang Sequence	CCCGCCCCCCGA
GC Content	67.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	P
Subcluster	P1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Arib1 (P1) Atcoo (P1) Bartholomew (P1) Bhagsy (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) Etoile (P1) FirstPlacePfu (P1) Fishburne (P1) Gavriela (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6)
Annotating Institution	Northwestern College
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	77
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Phineas_1 (terminase small subunit) Phineas_2 (terminase large subunit) Phineas_3 (portal protein) Phineas_4 (capsid maturation protease) Phineas_5 (scaffolding protein) Phineas_6 (major capsid protein) Phineas_7 Phineas_8 (head-to-tail adaptor) Phineas_9 (head-to-tail stopper) Phineas_10 Phineas_11 Phineas_12 Phineas_13 (tail terminator) Phineas_14 (major tail protein) Phineas_15 (tail assembly chaperone) Phineas_16 (tail assembly chaperone) Phineas_17 (tape measure protein) Phineas_18 (minor tail protein) Phineas_19 (minor tail protein) Phineas_20 (minor tail protein) Phineas_21 (minor tail protein) Phineas_22 (minor tail protein) Phineas_23 Phineas_24 Phineas_25 Phineas_26 (lysin A) Phineas_27 (lysin B) Phineas_28 Phineas_29 Phineas_30 (tyrosine integrase) Phineas_31 (immunity repressor) Phineas_32 (excise) Phineas_33 Phineas_34 (antirepressor) Phineas_35 Phineas_36 Phineas_37 Phineas_38 Phineas_39 Phineas_40 Phineas_41 Phineas_42 Phineas_43 Phineas_44 Phineas_45 (HNH endonuclease) Phineas_46 Phineas_47 (RecB-like exonuclease/helicase) Phineas_48 (RecT-like DNA pairing protein) Phineas_49 Phineas_50 Phineas_51 (FtsK-like DNA translocase) Phineas_52 (RusA-like resolvase) Phineas_53 Phineas_54 Phineas_55 (DNA methylase) Phineas_56 Phineas_57 (DNA methylase) Phineas_58 Phineas_59 (helix-turn-helix DNA binding domain protein) Phineas_60 Phineas_61 Phineas_62 Phineas_63 Phineas_64 Phineas_65 Phineas_66 (helix-turn-helix DNA binding protein) Phineas_67 Phineas_68 Phineas_69 Phineas_70 Phineas_71 Phineas_72 Phineas_73 (ribbon-helix-helix DNA binding domain protein) Phineas_74 Phineas_75 Phineas_76 Phineas_77 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK820639
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	147
Pitt Freezer Box Grid#	D11
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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