| Detailed Information for Phage Polkaroo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Amy Stewart |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 19°C |
| GPS Coordinates | 45.240719 N, 75.38586 W Map |
| Discovery Notes | Found in a wet garden bed planted outside of the uOttawa Medical campus at RGN |
| Naming Notes | Named after the mysterious Polkaroo from "The Polkadot Door" TV show as it was a creature that was hard to catch. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1920 |
| Genome length (bp) | 49084 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | Small plaques, clear, small halo. |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
154 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
