The Actinobacteriophage Database

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Detailed Information for Phage Purky
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Taylor Purks
Year Found	2013
Location Found	Washington, DC USA
Finding Institution	Howard University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	38.922244 N, 77.022039 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 31, 2014
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	867
Genome length (bp)	50513
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CCTGCCGCCCG
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	P
Subcluster	P6
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Arib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6)
Annotating Institution	College of Charleston
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	84
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Purky_1 (terminase small subunit) Purky_2 (terminase large subunit) Purky_3 (portal protein) Purky_4 (capsid maturation protease) Purky_5 (scaffolding protein) Purky_6 (major capsid protein) Purky_7 Purky_8 (head-to-tail adaptor) Purky_9 (head-to-tail stopper) Purky_10 Purky_11 Purky_12 (tail terminator) Purky_13 (major tail protein) Purky_14 (tail assembly chaperone) Purky_15 (tail assembly chaperone) Purky_16 (tape measure protein) Purky_17 (minor tail protein) Purky_18 (minor tail protein) Purky_19 Purky_20 (minor tail protein) Purky_21 Purky_22 Purky_23 Purky_24 Purky_25 Purky_26 (lysin A) Purky_27 (holin) Purky_28 Purky_29 Purky_30 (helix-turn-helix DNA binding protein) Purky_31 Purky_32 Purky_33 Purky_34 Purky_35 Purky_36 (excise) Purky_37 (tyrosine integrase) Purky_38 (immunity repressor) Purky_39 (excise) Purky_40 Purky_41 (antirepressor) Purky_42 Purky_43 Purky_44 Purky_45 Purky_46 Purky_47 Purky_48 Purky_49 Purky_50 Purky_51 (RecB-like exonuclease/helicase) Purky_52 (RecT-like DNA pairing protein) Purky_53 Purky_54 Purky_55 (FtsK-like DNA translocase) Purky_56 (RusA-like resolvase) Purky_57 (DNA methylase) Purky_58 Purky_59 Purky_60 Purky_61 Purky_62 Purky_63 Purky_64 Purky_65 Purky_66 Purky_67 Purky_68 Purky_69 Purky_70 Purky_71 Purky_72 Purky_73 Purky_74 Purky_75 (RepA-like replication initiator) Purky_76 Purky_77 Purky_78 (helix-turn-helix DNA binding protein) Purky_79 Purky_80 Purky_81 Purky_82 Purky_83 Purky_84 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN096355
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	44
Pitt Freezer Box Grid#	B12
Available Files
GenBank File for Phamerator	Download

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