| Detailed Information for Phage Pygmy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Benjamin M Hughes and Dylan W Stanley |
| Year Found | 2023 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.464444 N, 94.725556 W Map |
| Discovery Notes | Phage Pygmy was isolated from a soil sample collected at 4:35 pm CDT on Tuesday August 22, 2023 from near the edge of the pool at LeTourneau University in Longview, Texas, USA. The location was well covered with green grass. The hole that was dug was 8cm wide and 4cm deep, and 5g of soil from it was ultimately used. The soil was dark, damp and loose. The weather was sunny with a temperature of 39.4°C. |
| Naming Notes | Pygmy means very small |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 30, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1762 |
| Genome length (bp) | 48612 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques looked small and clear (albeit being a temperate phage), with smooth edges and had an average diameter of 1.4 mm (range 0.9 – 2.2 mm, n = 10). |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP758913 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.9 x 10^10 PFU/ml |
| Pitt Freezer Box# |
168 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |