| Detailed Information for Phage Simpson |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Mariam Mahran |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 18°C |
| GPS Coordinates | 45.2636 N, 75.71769 W Map |
| Discovery Notes | This soil sample was collected from the river bed of Jock River. The soil was dark and damp from its proximity to water and from the rain in the past several days. |
| Naming Notes | This bacteriophage was named after my cat, Simpson. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1746 |
| Genome length (bp) | 43853 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | The plaques for Simpson are large. They also have a bullseye, indicating that this bacteriophage has a lysogenic cycle. |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
154 |
| Pitt Freezer Box Grid# |
H4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
