| Detailed Information for Phage Triste |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Caroline Chou and Hannah Mitchell |
| Year Found | 2019 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 39°C |
| GPS Coordinates | 38.636389 N, 90.293611 W Map |
| Discovery Notes | In Forest Park, next to a big tree, lots of undergrowth, insects, decomposing leaves, plant roots around, dug 10cm in to the soil, soil crumbly but moist. |
| Naming Notes | Pronounced "Treest"
Triste means sad in French. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 20, 2020 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 101 |
| Genome length (bp) | 51568 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGCCAGTGAT |
| GC Content | 65.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | around 3mm in diameter, sometimes had red ring of bacterial contamination outlining it, slightly turbid (became less turbid as more rounds of purification were done) |
| Morphotype | Siphoviridae |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ648040 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
