| Detailed Information for Phage Unstoppable |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Jasmine Hatter |
| Year Found | 2020 |
| Location Found | Denton, TX United States of America |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.212569 N, 97.14687 W Map |
| Discovery Notes | Collected soil in Denton, Texas. The temperature was about 100 degrees F and it was muddy from the sprinklers from earlier in the morning. The location is under a tree near al dorm. I dug about the top 1 inch of soil from between the tree roots and placed in a plastic bag. |
| Naming Notes | My Phage can not be beat, so I thought it would great to name it "unstoppable" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1778 |
| Genome length (bp) | 49556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The phage are clear and circular with an average diameter of 2 mm. |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OL455894 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8*10^9 pfu/mL |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
B5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
