| Detailed Information for Phage VieEnRose |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Savannah Schultz-Loggie |
| Year Found | 2019 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.215092 N, 97.148592 W Map |
| Discovery Notes | Collected Soil Sample underneath an unknown tree in the parking lot of CVS at 8:26pm. The soil was in a flower bed that had trees and an ivy like short bush plant that covered all the soil except for where the soil sample was taken. The soil was not very rich and moist, but it was still a dark color. It is very fine soil, and there were pieces of trash (Cheetos bag and other unidentifiable wrappers) near the collection site. Dug down an inch with a clean spoon and then collected the soil using the spoon to put about 5mL in a 14mL test tube. Outside temperature was 88°F or 31.1°C. It was clear skies and dark outside, and the sun had just set and it was not windy. GPS coordinates for the sample are N 33° 12' 54.331", W 97° 8' 54.931". |
| Naming Notes | I named my phage after one of my favorite songs. The name of the sone is La Vie En Rose by Édith Piaf. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 26, 2021 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 94 |
| Genome length (bp) | 50193 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCTGCGTCAT |
| GC Content | 68.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD6 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Negative for lysogeny test |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | It's circular and clear and has a diameter of .8 cm. |
| Number of Genes | 78 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON526983 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.4x10^8 pfu/mL |
| Pitt Freezer Box# |
104 |
| Pitt Freezer Box Grid# |
D5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
