| Detailed Information for Phage Yosif |
| Discovery Information |
| Isolation Host | Streptomyces toxytricini NRRL B-5426 |
| Found By | Hessah alzaid |
| Year Found | 2016 |
| Location Found | Housten, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.757 N, 95.35775 W Map |
| Discovery Notes | My friend collected soil sample for me in Winter 2015. It was wet. |
| Naming Notes | the name is related to my husband. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 9, 2016 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 700 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 50129 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCATGTGAT |
| End Determination Notes | Ends were PCRed across to acquire the overhang sequence, which is high-quality. However, since this phage was sequenced using a Nextera transposon-mediated library prep protocol, the exact start and end of the genome are not apparent from the reads. We assumed that this phage would have the same length overhang as its nearest relatives, Amela and Verse, hence the 11 bp overhang claim. |
| GC Content | 66.1% |
| Sequencing Notes | There are a couple of interesting variations in this population. First, approximately 45% of the reads have a T instead of the published C at position 45004. Second, around half of the reads indicate a 1321 bp deletion from position 47143 to position 48462, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | It is small and clear. The diameter is 0.9 millimetre. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH248947 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^8 pfu/mL |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
