| Detailed Information for Phage Bartholomew |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Fallert, Lucinda Krenzke, Dezerene Thompson |
| Year Found | 2014 |
| Location Found | Kenosha, WI USA |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.619444 N, 87.821389 W Map |
| Discovery Notes | The soil sample was taken at night from the dirt behind a shrub outside Madrigrano Residence Hall. The soil was taken at a depth of one inch and the temperature was 68 degrees F. |
| Naming Notes | Because our phage's plaques have a distinct halo, we named it after the Catholic saint Bartholomew. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 150 |
| Genome length (bp) | 46484 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Bartholomew is temperate and has two distinct morphologies. Approximately 85% of the plaques on a given plate display a morphology that is 2mm in size and has a distinct halo with a clear center. The other morphology is about 1mm in size and appears as a pinprick. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140399 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.4e8 pfu/mL |
| Date of SEA Lysate Titering |
Nov 14, 2015 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
