| Detailed Information for Phage BenoitCattle |
| Discovery Information |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Rece Buckmaster |
| Year Found | 2023 |
| Location Found | Esbon, KS United States |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 34°C |
| GPS Coordinates | 39.78215 N, 98.40191 W Map |
| Discovery Notes | This sample was colleced from a farm pond in rural Jewell County in Kansas. The sample was collected at 7:00 p.m. on September 4, 2023.
The water sample was enriched using Gordonia. |
| Naming Notes | The sample was taken from the pond of a cattle ranch that belonged to Benoit Angus. I had to honor them for allowing me to use their resources to find my phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 887 |
| Genome length (bp) | 77049 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 58.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage was lytic based upon lysogeny testing. |
| Other Cluster Members |
|
| Annotating Institution | Kansas State University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques generally are isolated, large, smooth margined and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184834 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.6*10^9 |
| Pitt Freezer Box# |
177 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
