The Actinobacteriophage Database

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Detailed Information for Phage Donovan
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Chantelle Willette and Seth Stevens
Year Found	2012
Location Found	Mineral Wells, TX USA
Finding Institution	University of North Texas
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	32.75915 N, 98.069478 W Map
Discovery Notes	Area watered nightly 96 degrees Fahrenheit 3 cm below surface
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 10, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Ion Torrent
Approximate Shotgun Coverage	660
Sanger Finishing Reactions	4
Genome length (bp)	47162
Character of genome ends	3' Sticky Overhang
Overhang Length	12 bases
Overhang Sequence	CCTGCCCCCCGA
GC Content	67.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	P
Subcluster	P1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Arib1 (P1) Atcoo (P1) Bartholomew (P1) Bogie (P1) Brusacoram (P1) Bunnies (P1) CactusJack (P1) Camster (P1) Donovan (P1) Dynamo (P1) FirstPlacePfu (P1) Fishburne (P1) Glaske (P1) GreaseLightnin (P1) HUHilltop (P1) Jebeks (P1) Jung (P1) Juniormint (P1) Kari (P1) KilKor (P1) Ksquared (P1) Langerak (P1) Majeke (P1) Malithi (P1) Mangethe (P1) Megiddo (P1) Necropolis (P1) Phalm (P1) Phegasus (P1) Phineas (P1) Polkaroo (P1) Pygmy (P1) Shipwreck (P1) Sonah (P1) StevieRay (P1) StressBall (P1) Techage (P1) Thespis (P1) Venti (P1) Vidya (P1) Willsammy (P1) Zilizebeth (P1) Tortellini (P2) Xavia (P3) BigNuz (P4) Nazo (P4) Phayonce (P5) ThulaThula (P5) Purky (P6)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Small, clear plaques. Generally no larger than 1 mm in diameter.
Morphotype	Siphoviridae
Number of Genes	78
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Donovan_1 (terminase small subunit) Donovan_2 (terminase large subunit) Donovan_3 (portal protein) Donovan_4 (capsid maturation protease) Donovan_5 (scaffolding protein) Donovan_6 (major capsid protein) Donovan_7 Donovan_8 (head-to-tail adaptor) Donovan_9 (head-to-tail stopper) Donovan_10 Donovan_11 Donovan_12 Donovan_13 (tail terminator) Donovan_14 (major tail protein) Donovan_15 (tail assembly chaperone) Donovan_16 (tail assembly chaperone) Donovan_17 (tape measure protein) Donovan_18 (minor tail protein) Donovan_19 (minor tail protein) Donovan_20 (minor tail protein) Donovan_21 (minor tail protein) Donovan_22 (minor tail protein) Donovan_23 Donovan_24 Donovan_25 Donovan_26 (lysin A) Donovan_27 (lysin B) Donovan_28 Donovan_29 Donovan_30 (tyrosine integrase) Donovan_31 (immunity repressor) Donovan_32 (excise) Donovan_33 Donovan_34 (antirepressor) Donovan_35 Donovan_36 Donovan_37 Donovan_38 Donovan_39 Donovan_40 Donovan_41 Donovan_42 Donovan_43 Donovan_44 Donovan_45 Donovan_46 (RecB-like exonuclease/helicase) Donovan_47 (RecT-like DNA pairing protein) Donovan_48 Donovan_49 (FtsK-like DNA translocase) Donovan_50 (RusA-like resolvase) Donovan_51 Donovan_52 Donovan_53 Donovan_54 Donovan_55 (helix-turn-helix DNA binding domain protein) Donovan_56 Donovan_57 (DNA methylase) Donovan_58 Donovan_59 Donovan_60 Donovan_61 Donovan_62 (RepA-like replication initiator) Donovan_63 Donovan_64 Donovan_65 Donovan_66 Donovan_67 Donovan_68 Donovan_69 Donovan_70 Donovan_71 Donovan_72 Donovan_73 Donovan_74 (ribbon-helix-helix DNA binding domain protein) Donovan_75 Donovan_76 Donovan_77 Donovan_78
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KF841477
Refseq Number	NC_023552
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Lysate Titer	2E11 pfu/mL
Date of SEA Lysate Titering	Oct 24, 2012
Available Files
Restriction Digest Picture	Download

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