| Detailed Information for Phage Harambe |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Corbin Fry |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.445986 N, 79.953236 W Map |
| Discovery Notes | The soil was collected on a sunny partly cloudy day with dry weather on the past 24 hours. The gps coordinates were N 40° 15' 52.4622" W -79° 34' 16.3992". This soil will be used to isolate possible phages in it through direct and enriched isolation. The soil was collected in a zip-lock bag outside of Clapp Hall at the University of Pittsburgh. |
| Naming Notes | This is named after a pet. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1160 |
| Genome length (bp) | 73511 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 184 bp |
| GC Content | 59.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic Phage |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Small clear circluar plaques |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450430 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.0 x 10^9 |
| Date of SEA Lysate Titering |
Oct 26, 2016 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
E2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
