| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Samantha J Pochiro |
| Year Found | 2018 |
| Location Found | Henderson, NV USA |
| Finding Institution | College of Southern Nevada |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.076304 N, 115.002681 W Map |
| Discovery Notes | •The sample was collected at the City of Henderson Bird Viewing Preserve along the north east side of pond number 2
•The coordinates of the location is: 36.076304, -115.002681
•The sample was dry and had a lot of small twigs or broken root parts in it. I dug about an inch or two below the surface until I saw darker sand underneath the surface.
•It was collected in Henderson, NV on 9/5/18. |
| Naming Notes | Jellybones was named after a song from the band The Unicorns. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1592 |
| Genome length (bp) | 77514 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 191 bp |
| GC Content | 59.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CS |
| Subcluster | CS2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of Southern Nevada |
| Annotation Status | In GenBank |
| Plaque Notes | The phage produced small plaques ranging from 1/2mm to 1mm in size with distinct borders |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN444874 |
| Refseq Number | None yet |
| Published in Paper | Harrington et al 2020, Microbiology Resource Announcements: Genome Sequences of Gordonia rubripertincta Bacteriophages Jellybones and NHagos |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
80 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
