| Detailed Information for Phage Legacy |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Jermiah Crowder |
| Year Found | 2020 |
| Location Found | The Colony, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.0486 N, 96.9191 W Map |
| Discovery Notes | Using Protocol 5.1 Collecting Environmental Samples, soil was collected from The Colony, Texas (33.0486°N, 96.9191°W) in order to isolate a bacteriophage. The air temperature surrounding the soil at time 17:28 when soil was retrieved was 93°F. The soil appeared a little moist around below the surface while at the surface, it was dry and flaky (Figure 0). Many organisms such as worms and other insects were found near the soil collection site. I dug 4 cm below the surface to collect the soil and placed it with a clean spoon into a plastic sandwich bag. The sample was named "Colony" soil. |
| Naming Notes | I chose Legacy because I have been obsessed with the name since I was in middle school. I plan to name my first born daughter Legacy and I want her to know that she was named after the phage I discovered. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 122 |
| Genome length (bp) | 49941 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Cabrini University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage is clear and circular with an average diameter of 1 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON208832 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4x10^9 pfu/mL |
| Pitt Freezer Box# |
117 |
| Pitt Freezer Box Grid# |
H1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |