| Detailed Information for Phage London |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Jasmae Flowers |
| Year Found | 2019 |
| Location Found | Chesapeake, VA United States of America |
| Finding Institution | Virginia State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.78 N, 76.275 W Map |
| Discovery Notes | I adopted the phage that I used while I was conducting my experiments from my classmate Demesha because I did not discover any phage of my own in the soil samples that I collected. The sample was collected in the front yard of her home in Chesapeake, Virginia. The temperature was 76 degrees Fahrenheit, the humidity was 72%, and it was a cloudy day.
-This phage was further characterized by graduate student, H. L. Hatke (2021)
-Confirmed to infect A. globiformis str. 2880 and NOT str. 2997 in June 2023. -A.R.Beyer |
| Naming Notes | The name of my phage came from a list of potential children names that I keep in my notes on my cellphone. I choose the name London, because London is the name that I want to name my future child and my phage is kind of like my child. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2712 |
| Genome length (bp) | 43599 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | AZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia State University |
| Annotation Status | In GenBank |
| Plaque Notes | London's plaque is composed of small circles that are about 0.5cm long. The plaque looks cloudy due to the fact that there are a bunch of small circles covering the whole plaque with agar in between most of them. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889366 |
| Refseq Number | None yet |
| Published in Paper | Beyer et al 2024, Microbiology Resource Announcements: Genome and characteristics of Arthrobacter globiformis AZ cluster phage London |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
106 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| GenBank File for Phamerator | Download |