| Detailed Information for Phage Majeke |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Siphenkosi Majeke |
| Year Found | 2015 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.817085 S, 30.941812 E Map |
| Discovery Notes | The soil sample was taken under a tree in E-block walkway |
| Naming Notes | The name of the phage comes from my last |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 7, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2104 |
| Genome length (bp) | 47612 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | immunity was observed on lysogen Rey and BigNuz |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | -The plaques were circular ring which looked like halos on the agar plate
-The halo shape formed after the 48 hours of incubation at 37 degrees Celsius.
-the size of the plaque was relatively medium size but a small portion of the plaques were smaller |
| Morphotype | Siphoviridae |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF472894 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
