Detailed Information for Phage Majeke |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Siphenkosi Majeke |
Year Found | 2015 |
Location Found | Durban, South Africa |
Finding Institution | University of Kwazulu-Natal |
Program | Mycobacterial Genetics Course, Durban, South Africa |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 29.817085 S, 30.941812 E Map |
Discovery Notes | The soil sample was taken under a tree in E-block walkway |
Naming Notes | The name of the phage comes from my last |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 7, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 2104 |
Genome length (bp) | 47612 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 12 bases |
Overhang Sequence | CCCGCCCCCCGA |
GC Content | 67.4% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | P |
Subcluster | P1 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | immunity was observed on lysogen Rey and BigNuz |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | -The plaques were circular ring which looked like halos on the agar plate -The halo shape formed after the 48 hours of incubation at 37 degrees Celsius. -the size of the plaque was relatively medium size but a small portion of the plaques were smaller |
Morphotype | Siphoviridae |
Number of Genes | 81 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MF472894 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Not in Pitt Archives |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |