| Detailed Information for Phage Megiddo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Katelyn Gutierrez and Marcus Hester |
| Year Found | 2018 |
| Location Found | Longview, TX USA |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.4624 N, 94.7262 W Map |
| Discovery Notes | A solid soil sample was collected from the base of a flowered bush in the wooded area by south field. Soil was dark and moist with tiny roots and wood pieces. On a warm morning the top 3-inch layer of soil and other natural matter was dug through with a plastic spoon and the underlying layer of soil was scooped into the plastic bag and sealed.
Ambient Temperature: 27 degrees Celsius
Time: 10:41 AM October 4, 2018 |
| Naming Notes | Megiddo is the Hebrew word for Armageddon, the site of the final end of times battle as written in the book of Revelation. Phage therapy reminds me of the idea of destruction as a means of restoration and redemption. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 339 |
| Genome length (bp) | 48783 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCTGCCGCCCCGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN807249 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
